Utilization of 3-chloro-2-methylbenzoic acid by Pseudomonas cepacia MB2 through the meta fission pathway

Article Abstract:

Growth of Pseudomonas cepacia strain MB2 using 3-chloro-2-methylbenzoate as the sole carbon source was rendered possible by its metabolism of this substrate through the meta fission pathway, with a 4-chlorocatechol as an intermediate. This organism appears unable to use the ortho pathway. The 4-chlorocatechol was a substrate for meta pyrocatechase, a hydrolase, which generated a non-toxic semialdehyde product. The hydrolase appears to be an essential control point, as its specificity is limited, thereby controlling further metabolism of the products of ring fission.

---

Degradation of 2-methylbenzoic acid by Pseudomonas cepacia MB2

Article Abstract:

Pseudomonas cepacia MB2, a microorganism that uses 2-methylbenzoic acid as its sole carbon source, was isolated and characterized. The strain thrived on benzoate, all mono- and dimethyl benzoates except 2-5- and 2, 6-dimethylbenzoates. However it failed to grow in chlorobenzoates without the methyl group. A mechanism for the degradation of methylbenzoates was proposed. This involves either dioxygenation to a carboxy-hydrodiol to form a catechol 3-methylcatechol or monoxygenation of the methyl group to form an intermediate that lactonized to isobenzofuranone.

Benzoic acid

---

Biodegradation of 2,4-dichlorophenol through a distal meta-fission pathway

Article Abstract:

Alcaligenes eutrophus JMP222 is a derivative of Alcaligenes eutrophus JMP134 that can metabolize 2,4-dichlorophenoxyacetic acid (2,4-DCP) via a meta-fission pathway by liberating chloride. The Alacaligenes eutrophus JMP222 grows on phenol and 2-methylphenol which mediated the meta-cleavage pathway. Resting-cell suspensions with omicron-cresol as a sole carbon source also degraded 2,4-DCP and liberated chloride to produce a ring fission product.

Microbiological synthesis, Bacterial genetics

Similar abstracts:

• Abstracts: Degradation of trichloroethylene by Pseudomonas cepacia G4 and the constitutive mutant strain G4 5223 PR1 in aquifer microcosms
• Abstracts: Utilization of cyanide as a nitrogenous substrate by Pseudomonas fluorescensNCIMB 11764: evidence for multiple pathways of metabolic conversion
• Abstracts: Effects of chlorobenzoate transformation on the Pseudomonas testosteroni biphenyl and chlorobiphenyl degradation pathway
• Abstracts: Initial hydrogenation during catabolism of picric acid by Rhodococcus erythropolis HL 24-2. part 2 Initial hydrogenation and extensive reduction of substituted 2,4-dinitrophenols
• Abstracts: Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp. strain M. Isolation and characterization of cesium-accumulating bacteria

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.