Utilization of cyanide as a nitrogenous substrate by Pseudomonas fluorescensNCIMB 11764: evidence for multiple pathways of metabolic conversion

Article Abstract:

The metabolism of cyanide by Pseudomonas fluorescens NCIMB 11764 was studied. Growth of P. fluorescence with cyanide as the sole nitrogen source was accomplished by using a batch-fed procedure, wherein cyanide was added at carefully timed intervals. Analysis of the metabolites showed that cyanide is is converted to carbon dioxide and ammonia. In addition, higher concentrations of cyanide were converted to formamide and formate, which were also formed during prolonged anaerobic incubation. The results indicate that P. fluorescence NCIMB 11764 can utilize cyanide via two metabolic pathways, one in presence of oxygen, and the other, under anaerobic conditions.

---

Accumulation of alpha-keto acids as essential components in cyanide assimilation by Pseudomonas fluorescens NCIMB 11764

Article Abstract:

Research was conducted to examine the accumulation of alpha-ketoglutarate acids as important components of cyanide assimilation by Pseudomonas fluorescens NCIMB 11764. Results demonstrate that cyanide loss from cultures independent of the one catalyzed by CNO takes place through a chemical reaction with excreted alpha-keto acids. Findings provide a foundation for a new model of cyanide utilization wherein alpha-keto takes on a critical role.

Ammonia

---

Enzymatic assimilation of cyanide via pterin-dependent oxygenolytic cleavage to ammonia and formate in Pseudomonas fluorescens NCIMB 11764

Article Abstract:

Research describes purification and characterization of cyanide oxygenase involved in cyanide assimilation by Pseudomonas fluorescens NCIMB 11764. Results demonstrate cyanide is enzymatically converted to formate and ammonia by a pterin-dependent oxygenative mechanism, with ammonia serving as the nitrogen source to the bacterium.

United States, Science & research, Oxidases, Microbial enzymes

Similar abstracts:

• Abstracts: Identification of an emergent and atypical Pseudomonas viridiflava lineage causing bacteriosis in plants of agronomic importance in a Spanish region
• Abstracts: Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae
• Abstracts: Stereospecific oxidation of (R)- and (S)-1-indanol by naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4
• Abstracts: Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium
• Abstracts: Inactivation of poliovirus type 1 in mixed human and swine wastes and by bacteria from swine manure. Degradation of Giardia lamblia cysts in mixed human and swine wastes

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.