PCR-ribotyping of Xenorhabdus and Photorhabdus isolates from the Caribbean region in relation to the taxonomy and geographic distribution of their nematode hosts

Article Abstract:

A polymorphism analysis of polymerase chain reaction-amplified 16S rRNA genes was used to study the genetic diversity of Xenorhabdus and Photorhabdus bacteria associated with entomopathogenic nematodes. The 117 bacteria strains analyzed were isolated from the Caribbean region. The genus Xenorhabdus was distinguished from the genus Photorhabdus using cluster analysis. Results reveal that the genus Xenorhabdus is more diverse than the genus Photorhabdus. Moreover, the bacterial genotype diversity of both genera is congruent with the host-nematode taxonomy.

Genotype, Genotypes, Polymerase chain reaction, Nematoda, Nematodes

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Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis

Article Abstract:

Transmission electron microscopy was combined with densitometric image analysis to develop a new method for measuring the dry weight of individual, natural bacteria. The new approach was used to quantify the dry weight as well as the cell volume of Escherichia coli and bacterial populations of two lakes. It was found that the dry weight of the E. coli cells was between 83 to 1,172 femtograms, while volume was between 0.1 to 3.5 cubic micrometers. A functional allometric relationship between the dry weight and volume of bacteria was also found.

Measurement, Genetic aspects, Cells (Biology), Escherichia coli, Cells, Biomass, Densitometry

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New insights into the colonization and release processes of Xenorhabdus nematophila and the morphology and ultrastructure of the bacterial receptacle of its nematode host, steinernema carpocapsae

Article Abstract:

The results from epifluorescence, differential interference contrast and transmission electron microscopy is presented showing that Xenorhabdus nematophila colonizes a receptacle in the anterior intestine of the infective juvenile stage of Steinernema carpocapsae. The results suggest that bacterial motility is not required for movement out of the distal region of the receptacle.

Bacterial motility

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