Phosphorylation of p34(super cdc2) regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo

Article Abstract:

The regulation of mitotic centrosome separation and bipolar spindle formation by the human Eg5 (HsEg5) kinesin-related protein requires the phosphorylation of the Thr-927 HsEg5 residue by p34(super cdc2). Antibodies against HsEg5 suppress the migration of centrosomes and the formation of the bipolar cells. The cells are arrested in a monoastral microtubule array containing state. A mutation preventing phosphorylation at Thr-927 stops HsEg5 from binding to the centrosome.

Physiological aspects, Proteins, Phosphorylation, Spindle (Cell division), Spindle (Cytoplasm)

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The mTOR inhibitor RAD001 sensitizes tumor cells to DNA-damaged induced apoptosis through inhibition of p21 translation

Article Abstract:

The use of isogenic tumor cells lines expressing either wild-type mTOR cDNA or a mutant that does not bind RAD001 demonstrates that the effects of RAD001 are through inhibition of mTOR function. Findings indicate that molecular rationale for combining DNA damaging agents with RAD001, showing that a general effect on a major anabolic process may dramatically enhance the efficacy of an established drug protocol in the treatment of cancer patients with solid tumors.

Rapamycin

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DRAM, a p53-induced modulator of autophagy, is critical for apoptosis

Article Abstract:

Study is presented to show that p53-induces autophagy in a Damage-regulated autophagy modulator (DRAM)-dependent manner and while over-expression of DRAM alones causes minimal cell death, DRAM is essential for p53-mediated death. The analysis not only describes a stress-induced regulator of autophagy but also highlight the relationship of DRAM and autophagy to p53 function and damage-induced programmed cell death.

Analysis, Causes of, Risk factors, Cancer, Tumor proteins, Tumour proteins

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