Phylogenetic affiliation of soil bacteria that degrade aliphatic polyesters available commercially as biodegradable plastics

Article Abstract:

A study was conducted to examine soil bacteria capable of degrading biodegradable plastics. Three different soil samples were collected, suspended and diluted with sterile water. A single colony was washed and frozen-thawed twice by liquid nitrogen to support phylogenetic analyses. Results indicated patterns of substrate specificity with regard to degradation. Findings also showed that bacteria capable of degrading biodegradable polymers are phylogenetically diverse.

Analysis, Biodegradation, Bacteria, Soils, Soil analysis

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Soluble methane monooxygenase gene clusters from trichloroethlyene-degrading Methylomonas sp. strains and detection of methanotrophs during in situ bioremediation

Article Abstract:

Soluble methane monooxygenase (MMO) gene clusters in strains of trichloroethlyene-degrading Methylomonas sp. and methanotrophs detected in in situ bioremediation are discussed. Two trichloroethlyene (TCE)-degrading methanotrophic strains were isolated. The strains are characterized based on both morphological and chemotaxonomic aspects. The sMMO genes of the strains are analyzed. A gene probe for sMMO genes has been designed and tested.

Japan, Statistical Data Included, Environmental aspects, Genetic aspects, Bioremediation, Oxidases, Bacterial genetics, Trichloroethylene

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Reevaluation and reduction of a PCR bias caused by reannealing of templates

Article Abstract:

A study is conducted to reevaluate the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies. The results reveal that the template reannealing at the annealing step would not cause the bias, however, the preferential homoduplex formation during temperature decrease from denaturation to annealing step would cause the bias.

Science & research, Polymerase chain reaction, Messenger RNA

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