Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

Article Abstract:

An experiment is made to determine if the biosynthetic genes for the lipopeptide calcium-dependent antibiotic of Streptomyces coelicolor A3(2) can be cloned. In this study, a PCR-based technique was used to isolate peptide-synthetase-encoding DNA fragments from the bacteria's chromosome. Results confirm the cloning of the genes as seen from disruptions in calcium-dependent antibiotic production.

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The Streptomyces coelicolor A3(2) lipAR operon encodes an extracellular lipase and a new type of transcriptional regulator

Article Abstract:

The Streptomyces coelicolor A3(2) lipAR operon is discussed. It has been found that it encodes a new type of transcriptional regulator and an extracellular lipase. A lipase-deficient strain of S. coelicolor was generated. It was found to be slightly affected in production of the polyketide antibiotic actinorhodin.

United Kingdom, Genetic regulation, Genetic transcription, Operons, Transcription (Genetics), Lipase

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Genetic suppression analysis of non-antibiotic-producing mutants of the Streptomyces coelicolor absA locus

Article Abstract:

Non-antibiotic-producing mutants of the Streptomyces coelicolor absA locus are discussed relative to genetic suppression analysis. The locus was identified for mutations which uncoupled sporulation in it from antibiotic synthesis.

United States, Usage, Chromosome mapping, Microbial mutation

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