Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens
Article Abstract:
An analysis of the internal transcribed spacer 1 (ITS-1)-ITS 2 region in the ribosomal DNA of Trichoderma harzianum is presented. The study was done to find out if there are functional groups within the species that could give clues as to its usefulness as biological control agent. Trichoderma is a fungi species found mostly in soil and in decomposing organic matter. It has antibiotic properties and can be used against a wide range of plant pathogens.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Polymorphic restriction patterns of ribosomal internal transcribed spacers in the biocontrol fungus Puccinia carduorum correlate with weed host origin
Article Abstract:
Restriction patterns of internal transcribed spacer regions of ribosomal DNA of Puccinia carduorum isolates of Carduus acanthoides and Carduus thoermeri differ from those of Carduus tenuiflorus and Carduus pycnocephalus. The sequence variability in the internal transcribed spacer regions is capable of differentiating isolates of P. carduorum from 4 different weeed hosts at the subspecies level and categorize into 2 host groups.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast
Article Abstract:
Soil-extracted DNA was pure and could trace 10 to the 5th power Corynebacterium glutamicum pUN1 cells per gram of soil by Escherichia coli HB 101, 10 to the 4th power cells of Hansenula polymorpha per gram of soil by slot blot DNA-DNA hybridizations, and 10 cells of Hansenula polymorpha per gram of soil by polymerase chains reactions amplification of the mamalian marker gene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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