Plasmid transfer from Pseudomonas putida to the indigenous bacteria in alfalfa sprouts: characterization, direct quantification, and in situ location of tranconjugant cells

Article Abstract:

Research has been conducted on conjugative plasmid transfer from Pseudomonas putida to the indigenous bacterial community of alfalfa sprouts cultivated from the commercially available seeds. The authors have demonstrated that alfalfa spouts provide environment which allows efficient conjugant gene transfer between the colonizing bacteria, and that the tranconjugant formation occurs later.

Denmark, All Other Miscellaneous Crop Farming, Field crops, except cash grains, not elsewhere classified, Alfalfa, Cells (Biology), Plasmids, Pseudomonas putida, Cells, Genetic transformation

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Purification and properties of a feruloyl esterase involved in lignocellulose degradation by Aureobasidium pullulans

Article Abstract:

Research has been conducted on black yeast Aureobasidium pullulans which produces feruloyl esterase while growing on birchwood xylan. The authors describe the purification of feruloyl esterase via anion-exchange, ultrafiltration, gel filtration chromatography, and hydrophobic interaction.

Austria, South Africa, Slovakia, Usage, Polymers, Esterases, Biodegradation, Yeast fungi, Yeasts (Fungi), Anion exchangers (Biology), Lignin, Chromatography, Chromatographic analysis, Plant cell walls

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