Plasmids pIP419 and pIP421 from Bacteroides: 5-nitroimidazole resistance genes and their upstream insertion sequence elements

Article Abstract:

A study of the genetic composition of two different 5-nitroimidazole (5-Ni) resistance genes, nimC and nimD, from Bacteroides plasmids pIP419 and pIP421 reveals that the nimC and the nimD regulate the synthesis of polypeptides. The genetic composition is important because Bacteroides are still susceptible to 5-Ni antibiotics, yet resist treatment from beta-lactam antibiotics. The gene sequences nimC and nimD were found to have resistant determinate structures, the nucleotide sequences and an identical insertion element, similar to those of nimA and nimB. The insertion sequence triggers the formation of those resistance genes.

Insertion elements, DNA, DNA insertion elements, Episomes

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The terminal structures of linear plasmids from Rhodococcus opacus

Article Abstract:

The linear plasmids pHG201, pHG204 and pHG205 of the phytophatogenic Rhodococcus opacus were investigated to determine the necessity of terminal inverted repeats for maintenance and replication in linear plasmids. Results indicate that the termini of these plasmids are capped with protein. Further, although pHG204 contain an impressive terminal repeat, the termini of the other two linear plasmids bear little homology. This indicates that terminal repeats may not be essential to replication and maintenance.

Genetic aspects, Bacteria, Pathogenic, Pathogenic bacteria

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Identification of two linear plasmids in the actinomycete Planobispora rosea

Article Abstract:

Linear plasmids pPR1 and pPR2 have been identified in the actinomycete Planobispora rosea. P. Rosea produces the antibiotic GE2270. Strains without both plasmids continue to produce GE2270, and are also resistant to it. The plasmids are similar but there is no cross-hybridization between their telomeric regions. Their structural organizations is similar to Streptomyces linear replicons.

Observations, Microbiological research

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