PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti
Article Abstract:
Researchers describe four PCR primers that recognize the nodbox 4 locus and the mucR gene of Sinorhizobium meliloti. These regions are specific for this species and are highly conserved.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
Polychlorinated biphenyl rhizoremediation by Pseudomonas fluorescens F113 derivatives, using a Sinorhizobium melitoti nod system to drive bhp gene expression
Article Abstract:
Heterologous rhizobial nodulation (nod) promoters from Sinorhizobium meliloti and its regulatory systems are tested to drive the expression of the bhp operon in Pseudomonas fluorescens F113 derivatives. The analysis showed that nodbox 4 from Sinorhizobium meliloti has a high level of expression in F113 that is dependant on an intact nodD1 gene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Ionic stress and osmotic pressure induce different alterations in the lipopolysaccharide of a Rhizobium meliloti strain
Article Abstract:
The ability of Rhizobium meliloti to tolerate high concentrations of salt in marshes may be related to changes in its lipopolysaccharide (LPS) structure in response to salt stress. The EFB1 strain of the bacteria can grow in concentrations of NaCl as high as 500mM. Electrophoresis and antibody assays indicated that the LPS of this strain changed in response to ionic stress and osmotic stress.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Thiamine-auxotrophic mutants of Pseudomonas fluorescens CHA0 are defective in cell-cell signaling and biocontrol factor expression
- Abstracts: Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments
- Abstracts: Cultivation and in situ detection of a thermophilic bacterium capable of oxidizing propionate in syntrophic association with hydrogenotrophic methanogens in a thermophilic methanogenic granular sludge