Controlling instability in gacS-gacA regulatory genes during inoculant production of Pseudomonas fluorescens biocontrol strains

Article Abstract:

Researchers have developed a screening method for detecting mutant forms of Pseudomonas fluorescens that accumulate during inoculum production. These mutants have a mutation in the gacS and gacA genes and are unable to produce antimicrobial compounds.

Pests, Biological control, Industrial microbiology

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Thiamine-auxotrophic mutants of Pseudomonas fluorescens CHA0 are defective in cell-cell signaling and biocontrol factor expression

Article Abstract:

The aim of the present study is to characterize mutations that affect the expression of the rsmZ gene in strain CHA0. Transposon Tn5 is used to isolate Pseudomonas fluorescens CHA0 insertion mutants that expressed an rsmZ-gfp fusion at reduced levels.

Science & research, Analysis, Physiological aspects, Gene mutations, Gene mutation, Transposons

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Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains

Article Abstract:

Understanding the environmental factors that regulate the biosynthesis of antimicrobial compounds by disease-suppressive strains of Pseudomonas fluorescens is an essential step toward improving the level and reliability of their biocontrol activity. Liquid culture assays were used to identify minerals and carbon sources which had a differential influence on the production of the antibiotics 2,4-diacetylphloroglucinol, pyoluteorin, and pyrrolnitrin and the siderophores salicylic acid and pyochelin by the model strain CHAO, isolated from a disease-suppressive soil in Switzerland.

Antibiotics, Anti-infective agents, Environmental aspects, Soil microbiology, Physiological regulation, Biological control systems

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