Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure

Article Abstract:

Oligonucleotide probes were used for analysis of microbial community structure in activated sludge. Group-specific cell counts were determined by in situ hybridization with fluorescent rRNA-targeted oligonucleotide probes. The activated sludge samples were dominated by proteobacteria from the alpha, beta or gamma subclass. Cultivation-dependent shifts in community composition was demonstrated.

Analysis, Nucleic acid probes

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In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plants

Article Abstract:

Researchers studied the activity of Nitrospira in wastewater treatment plants using 16S rRNA gene sequence analysis, fluorescence in situ hybridization, confocal laser scanning microscopy, and computer-aided three-dimensional visualization. Nitrospira-like bacteria are the predominant nitrite oxidizers in wastewater treatment plants.

Water treatment plants, Nitrogen-fixing microorganisms

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Cultivation-independent, semiautomatic determination of absolute bacterial cell numbers in environmental samples by fluorescence in situ hybridization

Article Abstract:

Researchers used a combination of fluorescence in situ hybridization (FISH), confocal laser scanning microscopy, and digital image analysis to measure the number of ammonia-oxidizing bacteria in municipal nitrifying activated sludge. This is the first known use of this technique to quantify bacterial cells in aggregates or biofilms.

Measurement, Bacteria, In situ hybridization

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