Procaryotic expression of single-chain variable-fragment (scFV) antibodies: secretion in L-form cells of Proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in Escherichia coli

Article Abstract:

A study was conducted to analyze the expression of various antibody constructs in Proteus mirabilis L Vi and compare it with their expression in Escherichia coli JM109. DNA manipulations were carried out using conventional techniques while the E. coli K-12 strain JM109 was utilized as the cloning and expression host. Results indicated that the expression of the antibody constructs in L-form cells were superior than their expression in E. coli based on product yield.

Analysis, Cells (Biology), Cells, Antibodies

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Morphological and physiological changes induced by high hydrostatic pressure in exponential- and stationary- phase cells of Escherichia coli: relationship with cell death

Article Abstract:

The various morphological and physiological changes that occur in pressurized Escherichia coli cells that might correlate with cell death are examined. The study of changes in gross morphology, loss of membrane integrity, the loss of osmotic responsiveness, changes in the conformation of nucleic acids, and protein coagulation in cells is presented.

Science & research, Cell death, Morphology (Biology), Morphology

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