Proteins homologous to the Xenopus germ cell-specific RNA-binding proteins p54/p56 are temporally expressed in mouse male germ cells

Article Abstract:

Antibodies particular to the Xenopus oocyte cytoplasmic 6S mRNA-binding particle p54/p56 and antibodies that act against Xenopus germ cell DNA-binding protein FRG Y2, which distinguish two RNA-binding proteins of the mouse testes, were studied. These two RNA-binding proteins, that are apparently immunological and operational homologues of the Xenopus germ cell-specific RNA/DNA-binding proteins p54/p56/FRG Y2, develop complexes with stored mRNAs and exist in male germ cells.

Nucleic acids, Oocytes, Oocyte donation, Antibodies

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Dynamics of germ plasm localization and its inhibition by ultraviolet irradiation in early cleavage Xenopus embryos

Article Abstract:

The germ plasm forms cytoplasmic islands which seem fixed to the vegetal yolk mass in the early cleavage of Xenopus egg, but after rotation start to fuse together. The continuous processes of local fusing and periodic surface contraction waves (SCWs) are responsible for the gathering at the vegetal pole. Labeling of the germ plams with fluorescent dye DiOC6(3) allows observation of the movements in line embryos through time-lapse confocal scanning optical microscopy.

Germplasm resources

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Analysis of Wnt/engrailed signaling in Xenopus embryos using Biolistics

Article Abstract:

The Biolistics approach was used to study the wnt signaling cascade in Xenopus embryonic development, specifically proper mid/hindbrain development, in relation to the regulation of the functional pathway of Engrailed-1 expression. Using the Biolistics Particle Delivery System, it was shown that ectopic expression of Xwnt-1 and Xwnt-5C is essential for the activation of the Xenopus XEn-1 gene, but not XEn-2.

Genetic regulation, Xenopus

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