Purification and characterization of a phenoloxidase (laccase) from the lignin-degrading basidiomycete PM1 (CECT 2971)

Article Abstract:

The significant ligninolytic activity detected in the basidiomylete PMI culture supernatant was a phenoloxidase (laccase), and this activity was produced in defined or complex media, and appeared as two proteins hands in native gel electrophoresis preparations. Enzyme induction was absent after treatment with specific potential laccase inducers. The emzye produced is a monomeric glycoprotein comprising 6.5% carbohydrate with a molecular weight of 64,000. Spectroscopic analysis showed that it has four bound copper atoms, a type I copper, a type II copper and a type III binuclear copper.

Microorganisms

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Identification of a laccase gene family in the new lignin-degrading basidiomycete CECT 20197

Article Abstract:

The identification of a laccase gene family in the new lignin degrading basidiomycete CECT 20197 was investigated using the effluents from sugar cane by-product fermentation industry. The study selected lignin-degrading white rot fungus and demonstrated its ability to produce and secrete several potent isozymes with laccase activity which decolorizes the effluent of a sugar cane by-product. Results reveal that white rot fungus degrade lignin efficiently in the absence of laccase. Furthermore, the production of laccase is repressed by glucose.

Vascular system of plants, Plant vascular systems

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Analysis of xysA, a gene from Streptomyces halstedii JM8 that encodes a 45-kilodalton modular xylanase Xys1

Article Abstract:

The molecular structure and the mRNA of the xysA gene of Streptomyces halstedii JM8 is studied. The study attempts to find out how it binds to proteins, the effect of the carbon source and instances of homologous genes and proteins are also studied. S. halstedii JM8 was obtained from rotting straw and was chosen because it was a good source of cellulases and xylanases. The Xys1 gene is one of two bacterial xylanase genes that have been cloned in large amounts.

Genetic aspects, Enzymes, Streptomyces, Messenger RNA, Enzyme structure-activity relationships, Amino acid sequence, Amino acid sequencing, Plant proteins

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