Purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138

Article Abstract:

Beta-1,4-mannanase isolated from the marine bacterium Vibrio sp. strain MA-138 is an extracellular glycoprotein with a molecular mass of 49 kDa and has maximum activity at pH 6.5 and 40 degree celsius. Polyacrylamide gel electrophoresis shows that beta-1,4-mannanase is a monomer and forms oligosaccharides of different sizes on hydrolysis. Physicochemical and enzymatic properties of the enzyme are comparable to other microbial mannanases.

Usage, Observations, Glycoproteins, Gel electrophoresis

---

Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product

Article Abstract:

The cloning and expression of the beta-agarase C gene (agaC) from the marine bacterium Vibrio sp. strain PO-303 is described, and the recombinant enzyme (rAgaC) is characterized. The gene beta-agarase C is the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and is hence believed to belong to a novel glycoside hydrolase family.

Science & research, Microbial genetic engineering, Vibrio cholerae, Genetic variation

---

Cloning, sequencing, and expression in Escherichia coli of the new gene encoding beta-1,3-xylanase from a marine bacterium, Vibrio sp. strain XY-214

Article Abstract:

The beta-1,3-xylanase gene of Vibrio sp. strain XY-214 codes a protein of 460 amino acids with a molecular mass of 51,323 Da, according to researchers who expressed the gene in E. coli. The enzyme hydrolyzes beta-1,3-xylan, producing several xylooligosaccharides.

Vibrio

Similar abstracts:

• Abstracts: Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii
• Abstracts: Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus. Biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by Bacillus coagulans I(sub 4)
• Abstracts: Tripartite haemolysin BL: isolation and characterization of two distinct homologous sets of components from a single Bacillus cereus isolate
• Abstracts: Genetic characterization of accumulation of polyhydroxyalkanoate from styrene in pseudomonas putida CA-3. Transcriptional regulation of styrene degradation in Pseudomonas putida CA-3
• Abstracts: Rapid detection, identification, and enumeration of Escherichia coli cells in municipal water by chemiluminescent in situ hybridization

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.