Purification and characterization of an extracellular poly(L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K104-1

Article Abstract:

Findings are presented on the ideal conditions for the biodegradation of poly(L-lactic acid), or PLA, a material being developed as a biodegradable and renewable plastic. A purified enzyme derived from the K104-1 and K104-2 strains of the Amycolatopsis bacterium degraded both solid and emulsion films of high-molecular-weight PLA into lactic acid, with the optimum temperature between 55 to 60 degrees Celsius and the optimum pH at 9.5.

Japan, Statistical Data Included, Environmental aspects, Plastics, Biodegradation, Soil microbiology, Biodegradable plastics

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Cloning, expression and cell surface localization of Paenibacillus sp. strain W- 61 xylanase 5, a multidomain xylanase

Article Abstract:

The classification of the bacterium, Aeromonas caviae W-61 to Paenibacillus sp. strain W-61 is cloned and expressed on the basis of the nucleotide sequence of the 16S rRNA gene emended and the xyn5 gene encoding xylanese 5 (Xyn5) in Escherichia coli. Findings concluded that Paenibacillus sp. strain W-61 Xyn5 is a cell surface anchored modular xylanase possessing a functional cellulose binding module and SLH domains.

Ribosomal RNA

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Amylomaltase of Pyrobaculum acrophilum IM2 produces thermoreversible starch gels

Article Abstract:

The amylomaltase-encoding gene from the hyperthermophilic archaeon Pyrobaculum acrophilum IM2 was cloned and expressed in Escherichia coli, and the gene product (PyAMase) was characterized. Acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties.

Genetic research

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