Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli

Article Abstract:

The properties of alpha-galactosidase from the thermophilic bacterium Thermus sp. strain T2 are described. The enzyme is an octamer and is 70% homologous to the alpha-galactosidase from Thermus brockianus.

Bacteria, Thermophilic, Microbial enzymes, Thermophiles

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Purification and substrate specificities of two alpha-L-arabinofuranosidases from Aspergillus awamori IFO 4033

Article Abstract:

A study was conducted to analyze the purification and substrate characteristics of two alpha-L-arabinofuranosidases determined from the culture filtrate of Aspergillus awamori IFO 4033. The influence of pH on the activity and stability of alpha-L-AFases were obtained in a series of McIlvaine buffers. A culture filtrate was then dialyzed against deionized water. In addition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis was carried out in a polyacrylamide gel.

Aspergillus, Hydrogen-ion concentration

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Cloning and high-level expression of alpha-galactosidase cDNA from Penicillium purpurogenum

Article Abstract:

Research was conducted to examine the cloning of Penicillium purpurogenum alpha-Gal cDNA, its expression in Saccharomyces cerevisiae and the purification and characterization of the recombinant enzyme. The mature enzyme consisted of 419 amino acid residues with a molecular mass of 46,334 Da as shown by the deduced amino acid sequence of the alpha-GAl cDNA. The purified recombinant enzyme showed slightly higher specific activity and properties that were almost identical to those of the native enzymes from P. purpurogenum.

Genetic aspects, Cloning, Gene expression, Circular DNA, Saccharomyces cerevisiae

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