Purification and structural determination of an inhibitor of Starfish oocyte maturation from a Bacillus species

Article Abstract:

An oocyst maturation inhibiting substance (OMIS) obtained from a bacterial culture has been found to inhibit the 1-methyladenine-induced oocyte maturation but not the dithiothreitol-induced maturation. The OMIS is the lactone of 3-hydroxy-13-methyltetradecanoyl-Glu-Leu-Leu-Val-Asp-Leu-Leu. Though the amino acid configurations have not been determined, the structure and inhibition activity of this OMIS are the same as that of surfactin. OMIS inhibits the oocyte maturation by affecting either the hormone signal transduction or the cell cycle regulation.

Oocytes, Oocyte donation, Animal development, Starfishes

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Purification and properties of ArfI, an alpha-L-arabinofuranosidase from Cytophaga xylanolytica

Article Abstract:

A study was conducted to determine purification and properties of alpha-L-arabinofuranosidase from Cytophaga xylanolytica. Purification was undertaken via anion exchange and hydrophobic interaction column chromatography. Results revealed that ArfI demonstrated zero hydrolytic activity toward a range of p-nitrophenyl- or 4-methylumbelliferyl-glycosides apart from arabinoside. Furthermore, ArfI was generally cytoplasmic which connotes that its activity in vivo is most suitable to hydrolysis of arabinose-containing oligosaccharides.

Composition, Enzyme kinetics, Anaerobic bacteria, Furans, Vidarabine

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Production, purification, and characterization of a highly glucose-tolerant novel beta-glucosidase from Candida peltata

Article Abstract:

The beta-glucosidase produced and purified from Candida peltata hydrolyzes cellobiose, shows a synergistic interaction with cellulase and has high glucose tolerance. The enzyme is a monomeric protein with a molecular weight of 43,000, and has maximum activity at 50 degrees celsius and 5.0 pH. The beta-glucosidase has little transglucosylation activity. Divalent cations are unessential for the enzymatic activity which is unaffected by p-chloromercuribenzoate.

Glucosidases, Alpha glucosidases, Candida

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