Purification, characterization, and nucleotide sequence of an intracellular maltotriose-producing alpha-amylase from Streptococcus bovis 148

Article Abstract:

A research was conducted to study the purification and characterization of an intracellular alpha-amylase from Streptococcus bovis 148. Two alpha-amylase activities were detected together in the cell extract which resulted from the adherence of the extracellular enzyme to the carbohydrate capsular material surrounding S. bovis 148 cells. Moreover, the deduced amino acid sequence of the intracellular alpha-amylase was similar to the sequences of the amylase detected in the cell extract of S. bovis JB1.

Streptococcus, Amino acids, Amylases

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Biochemical characterization of chloromethane emission from the wood-rotting fungus Phellinus pomaceus

Article Abstract:

Research was conducted to examine the biochemical nature of the CH3Cl-evolving system of the wood-rotting fungus Phellinus pomaceus. It was characterized using a membrane fraction since various detergents failed to solubilize the enzyme in active form. The methylation by methyl chloride transferase was inhibited by S-adenosyl-L-homocysteine which suggests that S-adenosyl-L-methionine is a natural methyl donor.

Chloromethane, Methyl chloride, Halogens, Halogen elements

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Biochemical and molecular characterization of a laccase from Marasmius quercophilus

Article Abstract:

Researchers describe the characteristics of a laccase isolated from the white-rot fungus Marasmius quercophilus. It belongs to the blue copper oxidase family of enzymes, and has the potential to be used in bioremediation of xenobiotics in effluents.

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