Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution

Article Abstract:

Quantitative PCR assays are developed for the detection of bovine feces-specific genetic markers and a method is designed for the enumeration of these markers by using a Markov chain Monte Carlo approach. Acceptable assay performance combined with the prevalence of DNA targets across different cattle populations have shown that these quantitative assays are useful in monitoring bovine fecal pollution in ambient waters.

Waste Management and Remediation Services, Water Pollution, Analysis, Usage, Polymerase chain reaction, Monte Carlo method, Monte Carlo methods

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Single-nucleotide polymorphism phylotyping of Escherichia coli

Article Abstract:

A rapid and easily automated phylogenetic grouping technique based on analysis of bacterial genome single-nucleotide polymorphisms (SNPs) was developed. The results indicated that the information for the 13 SNPs are sufficient to construct the phylogenic history of Escherichia coli and that the SNP phylotyping approach are suitable and robust for phylogenic analyses of a wide variety of bacterial species.

Phylogeny, Single nucleotide polymorphisms

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Detection of genetic markers of fecal indicator bacteria in Lake Michigan and determination of their relationship to Escherichia coli densities using standard microbiological methods

Article Abstract:

Lake Michigan surface waters impacted by fecal pollution are assessed to determine the occurrence of genetic markers for Bacteroides and Escherichia coli. This analysis shows the usefulness of non-culture-based microbial-source tracking approaches and the prevalence of these genetic markers in the Great Lakes, including freshwater coastal beaches.

Michigan, Anaerobic bacteria, Lake Michigan

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