Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples

Article Abstract:

A quantitative reverse transcriptase PCR (qRT-PCR) assay specifically tailored to detect the viruses in environmental waters is described as human enteroviruses serves as a more accurate indicator of human fecal contamination than conventional bacteriological fecal indicators. The findings reveal that the ability to the assay to identify false negatives and provide improved quantitative assessments of enterovirus concentrations would assist the tracking of human fecal contamination and the assessments of potential public health risk due to enteroviruses in recreational and shellfish harvesting waters.

Reverse Transcriptase, Analysis, Polymerase chain reaction, Enteroviruses, Structure

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In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay

Article Abstract:

Clostridium botulinum type C is an anaerobic, spore-forming bacterium found naturally in the sediments of lakes and marshes. A nested PCR was devised for the detection of C. botulinum type C1 toxin gene in sediments obtained from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, indicating both the widespread distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.

Botulinum toxin, Botulinum toxins

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Sensitive detection of botulinum neurotoxin types C and D with an immunoaffinity chromatographic column test

Article Abstract:

A sensitive and specific immunoassay for the simultaneous detection of Clostridium botulinum type C (BoNT/C) and type and type D neurotoxin is developed. Streptavidin-poly-horseradish peroxidase is used as a conjugate and a precipitating substrate allowed the direct semiquantitative readout of the assay and for a more accurate quantitative detection, the substrate can be eluted and measured in a photometer.

Science & research, Enzymes for Immunoassay, Research, Methods, Immunoassay enzymes, Neurotoxic agents, Chromatography

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