Virulence genes in environmental strains of Vibrio cholerae

Article Abstract:

Polymerase chain analysis of diverse serotypes and ribotypes of environmental strains of Vibrio cholerae demonstrate the presence of virulence genes or their homologues in the strains. Data suggest that this feature forms a bsis for the existence of a environmental reservoir of V. cholerae virulence genes.

United States, Japan, India, Bangladesh, Statistical Data Included, Usage, Gene expression, Virulence (Microbiology), Environmental testing

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Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-O1 strains isolated from Calcutta, India by using a NAG-ST DNA probe

Article Abstract:

A recently developed heat-stable enterotoxin (NAG-ST) DNA probe was used to reassess a collection of environmental strains of Vibrio cholerae. The probe hybridized with 12 strains which were subdivided into three representative groups. Concentrated supernatants from these groups produced fluid accumulation in the suckling mouse assay even after heating. Enzyme-linked immunosorbent assay showed that the supernatants also inhibited antibody binding to test NAG-ST. A cholera toxin (CT) DNA probe was also used in the reassessment, detecting six strains. All of the CT-positive strains produced CT in vitro. All the NAG-ST- and CT-positive strains were hemolysin positive.

Environmental aspects, Enterotoxins, Calcutta, India, Cholera toxin

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Effect of Transport at ambient temperature on detection and isolation of Vibrio cholerae from environmental samples

Article Abstract:

A study was conducted to understand the effect of transporting environmental samples at the ambient air temperature on isolation and enumeration of Vibrio cholerae. The findings indicate that holding samples isolated from a freshwater ecosystem at ambient temperatures ranging from 31 degree Celsius to 35 degree Celsius for 20 hours did not have deleterious effects but rather facilitated the growth of the total heterotrophic bacteria.

Science & research

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