Receptor binding domain of Escherichia coli F18 Fimbrial adhesin FedF can be both efficienty secreted and surface displayed in a functional form in Lactococcus lactis

Article Abstract:

Various alternatives for cell surface presentation were studied for developing an efficient production system for the receptor binding domain of the Escherichia coli F18 fimbrial adhesin FedF in Lactococcus lactis. The results confirm that the receptor binding domain of the F18 adhesin can be produced, secreted and surface displayed in a functionally active form in Lactococcus lactis.

Escherichia coli

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Surface display of the receptor-binding region of the Lactobacillus brevis S-layer protein in Lactococcus lactis provides nonadhesive lactococci with the ability to adhere to intestinal cells

Article Abstract:

Research demonstrates the usage of a surface display system for the transfer and expression of the SlpA receptor-binding region in a nonadhesive Lactococcus lactis from an adhesive Lactobacillus brevis rendering the former to be able to adhere to human gut epithelial cell line and fibronectin through slpA adhesin.va

Finland, Analysis, Physiological aspects, Cell adhesion molecules, Cell adhesion, Genetic transformation

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Characterization of a mobile clpL gene from Lactobacillus rhamnosus

Article Abstract:

The probiotic Lactobacillus rhamnosus E-97800 carries two genes capable of encoding ClpL proteins. A demonstration that both of the clpL genes are heat induced and that mobilization of the clpL2 gene can be triggered during prolonged incubation at elevated temperature is presented.

Lactobacillus, Genetic research, Probiotics

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