Recruitment of ORC or CDC6 to DNA is sufficient to create an artificial origin of replication in mammalian cells

Article Abstract:

Origins of replication are expected to recruit initiation proteins like origin recognition complex (ORC), an Cdc6 in eukaryotes and provide a platform for unwinding DNA. Artificial origin recapitulates many of the regulatory features of physiological origins and is valuable for studies on replication initiation in mammalian cells and the artificial recruitment of a eukaryotic cellular replication initiation factor to a DNA sequence can create a functional origin of replication, providing a robust genetic assay for these factors and a novel approach to generating episomal vectors for gene therapy.

United States, Mammals

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Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading

Article Abstract:

The function of the Saccharomyces cerevisiae Orc6, which is the only origin recognition complex (ORC) subunit that is not required for DNA binding, is examined. The analysis of the Cdt1-ORC fusion has disclosed many important properties of prereplicative complex (pre-RC), which has also included the need for Cdt1 release to enable repeated Mcm2-7 loading.

Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Brewers' Yeast, DNA-ligand interactions, DNA binding, Brewer's yeast, Saccharomyces cerevisiae

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Interaction of the S-phase cylin Clb5 with an 'RXL' docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch

Article Abstract:

The study shows that the S-phase cyclin Clb5 binds stably and directly to the origin recognition complex (ORC). It is proposed that Clb5 binding to ORC provides an origin-localized replication control switch that specifically prevents reinitiation at replicated origins.

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