Recombinant SNAP-25 is an effective substrate for Clostridium botulinum type A toxin endopeptidase activity in vitro

Article Abstract:

A research on the composition of a recombinant fragment of SNAP-25 (synaptosomal-associated protein of molecular mass 25 kDA) spanning the toxin cleavage site and its function as an in vitro substrate for BoNT/A was conducted. The primary methodology used was subjecting the cDNA clone, pSNAP8.52 in a purified and therapeutic preparation. Findings showed that endopeptidase assay was more sensitive than the mouse bioassay in detecting toxin in recuperative mechanisms. Furthermore, the analysis that induced cultures regulate cytoplasmic extracts containing larger amounts of 49 kDA protein than uninduced cultures was also established.

Clostridium botulinum, Recombinant DNA, Toxins, Neurotoxic agents

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Attenuated typhoid vaccine Salmonella typhi Ty21a: fingerprinting and quality control

Article Abstract:

New quality control approaches are needed for live attenuated vaccines. To develop new testing for quality control, the attenuated vaccine strain Salmonella typhi Ty21a and its present strain Ty2 were characterized by pulsed-field gel electrophoresis (PFGE) and direct nucleotide sequence analysis. Fingerprints from the resolution on PFGE of chromosomal DNA was used to distinguish mutant and parent strains. These strains can also be distinguished by direct nucleotide sequence analysis of the galE gene.

Analysis, Genetic aspects, Study and teaching, Nucleotide sequence, Base sequence, Identification and classification, Gel electrophoresis, Genetic research, Genetic code, Salmonella typhosa, Salmonella typhi, Typhoid vaccine, Typhoid vaccines

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Monoclonal antibody recognition of members of the meningococcal P1.10 variable region family: implications for serological typing and vaccine design

Article Abstract:

The reactivity of eight members of the meningococcal P1.10 variable region family to monoclonal antibodies is investigated using enzyme-linked immunosorbent assay. The members of the meningococcal family are identified by nucleotide sequence analysis of porA genes of Neisseria meningitidis. Results indicate two levels of antigenic variation in porA genes. The implications of the results on the search for vaccines against meningococci are discussed.

Monoclonal antibodies, Enzyme-linked immunosorbent assay, Neisseria meningitidis

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