Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I

Article Abstract:

Researchers have used 3' terminal murine rDNA fused to an SV40 origin-based vector to reproduce the replication fork barrier (RFB) at the 3' end of eukaryotic ribosomal RNA genes. This barrier blocks bidirectional fork progression and restricts DNA replication to the same direction as transcription. It was found that mutations with the Sal box which impair the binding of the RNA polymerase I-specific transcription termination factor TTF-I also abolish RFB function. This indicates that TTF-I may be the trans-acting factor which prompts replication fork arrest.

Recombinant DNA

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Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor 1

Article Abstract:

A model system is designed with the Xenopus egg extracts and human cell extracts to examine DNA repair and chromatin assembly. In the former, repair of lesions occurs on an ultraviolet-treated plasmid together with chromatin assembly. A complementation assay is also demonstrated with human cell extracts that support DNA repair, with the incapacity for chromatin assembly remedied by Xenopus extract. The human chromatin assembly factor 1, which is responsible for the specificity of replicating DNA assembly, also supports chromatin assembly of repaired DNA.

DNA repair, Chromatin, Xenopus, Human cell culture

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ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome

Article Abstract:

A study of DNA replication in origin recognition complex (ORC) mutants binding to Saccharomyces cerevisiae under specific temperature conditions revealed that temperature influences the rate of replication in wild-type cells, orc2-1 ad orc5-1, with rate of replication decreasing as temperature increases. Use of genetic screening indicated CDC6 as the multicopy suppressor of orc5-1, 2-D gel and biochemical analyses suggested an interaction between Cdc6p and ORC.

Saccharomyces

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