Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite

Article Abstract:

Biological indicators employing luminescent recombinant bacteria are generated to detect the presence of arsenite, antimonite and cadmium. The gene for firefly luciferase is combined with the promoter for the multiple metal resistance gene of Staphylococcus aureus and inserted in a detector bacteria. A repressor gene is also included in the plasmid. The presence of metals in the environment induces the expression of luciferase and the bioindicator organism glows.

Measurement, Pollution, Indicators (Biology), Biological indicators

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Use of controlled luciferase expression to monitor chemicals affecting protein synthesis

Article Abstract:

Chemicals may be screened for their ability to inhibit protein synthesis in Escherichia coli by using a bioluminescence assay. Real-time in vivo measurements of light produced by E. coli strains expressing luciferase genes under an inducible promoter reliably indicated the activity of known protein synthesis inhibitors. Comparison of different luciferase genes revealed that the lucGR gene from the luminous click beetle Pyrophorus plagiophthalamus worked best.

Analysis, Proteins, Protein synthesis, Microbiological research

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Interactions between motile Escherichia coli and glass in media with various ionic strengths, as observed with a three-dimensional-tracking microscope

Article Abstract:

Microbiological research using a three-dimensional-tracking microscope shows that the bacteria Escherichia coli uses reversible adhesion to moves over glass. Sedimentation velocity tests illustrate that the organism activelymoves away from the glass surface after swimming for a few minutes. Interaction energies between a glass surface and E. coli cells are shown at three ionic strengths.

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