Reduction of exogenous ferric iron by a surface-associated ferric reductase of Listeria spp

Article Abstract:

The study investigated the reduction of exogenous ferric iron by Listeria (L) monocytogenes, a Gram-positive food-borne pathogen. Intact cells of bacteria reduced and solubilized the iron to the ferrous form when exposed to ferric iron. Reduction took place after direct contact between the bacteria and the iron sources. The ferric reductase activity was expressed by reference strain and clinical isolates of the bacteria. Listeria's surface associated ferric reductase system may be one component of a general iron scavenging mechanism which can be used in different environments.

Analysis, Oxidation-reduction reaction, Oxidation-reduction reactions, Listeria monocytogenes, Iron compounds

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Molecular analysis of an extracellular protease gene from Vibrio parahaemolyticus

Article Abstract:

A 1761 bp ORF, which encodes a protein containing 587 residues, is found in the prtVp gene encoding the extracellular protease in Vibrio parahaemolyticus. A 25 amino acid signal peptide and a 562 amino acid extracellular polypeptide form the deduced polypeptide. The molecular mass of the polypeptide is 63156 Da. The protease is probably a metalloenzyme containing Zn as its activity is suppressed by inhibitors of Zn and metals. The structure of the protease is 32% similar to that of Vibrio alginolyticus. Details of cloning and characterization of the gene are given.

Observations, Vibrio, Amino acid sequence, Amino acid sequencing, Proteolytic enzyme genes

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Rapid detection of polyhydroxyalkanoate-accumulating bacteria isolated from the environment by colony PCR

Article Abstract:

Results demonstrate that colony polymerase chain reaction and semi-nested PCR are rapid and highly accurate methods for the detection of polyhydroxyalkanoate accumulation in bacteria. Data further indicate that the techniques are applicable to screen large number of environmental isolates of Ralstonia eutropha.

Taiwan, Usage, Testing, Environmental aspects, Bacteria, Polymerase chain reaction, Organic compounds

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