Repair and enterotoxin synthesis by Staphylococcus aureus after thermal shock

Article Abstract:

The effects of four liquid repair media (1% powdered skim milk in distilled water (PSM), complex medium, M9 minimal salt medium and saline solution) on the repair and enterotoxin production by four heat-shocked Staphylococcus aureus strains (FRI-100, FRI-137, FRI-472 and S6) were studied. Salt tolerance was used as a measure of the repair of damaged cells. PSM was the most efficient medium for promoting the repair of stressed cells. Enterotoxins were observed in all strains after 10 h.

Physiological aspects

---

In vitro assay of Staphylococcus aureus enterotoxin A activity in food

Article Abstract:

An alternative assay for Staphylococcus aureus enterotoxin A (SEA) activity in food, utilizing T-cell proliferation, was developed. Human and rat lymphocytes proliferate in response to concentrations of SEA as low as 1 pg/ml, well below the pathogenic dose of 100 ng. This proliferation assay is highly sensitive, quantitative and simple. Results showed that an in vitro cell proliferation assay is an advantageous substitute for current animal assays for measuring SEA activity in food.

---

Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods

Article Abstract:

A real-time immunoqunatitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. IqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods and no cross reactivity with SE other than SEB was observed.

Science & research

Similar abstracts:

• Abstracts: rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7
• Abstracts: Stable low-copy-number Staphylococcus aureus shuttle vectors. Analysis of the beta-glucoside utilization (bgI) genes of Shigella sonnei: evolutionary implications for their maintenance in a cryptic state
• Abstracts: Characterization of the genome of the Rhodococcus rhodochrous bacteriophage NJL. Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous
• Abstracts: Wintering shorebirds increase after kelp (macrocystis) recovery. Demography of a California least tern colony including effects of the 1982-1983 El Nino
• Abstracts: Isolation and entomotoxic properties of the Xenorhabdus nematophilus F1 lecithinase. Conditional suicide system of Escherichia coli released into soil that uses the Bacillus subtilis sacB gene

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.