Resistance of Spiroplasma citri lines to the virus SVTS2 is associated with integration of viral DNA sequences into host chromosomal and extrachromosomal DNA

Article Abstract:

Incorporation of fragments of the spiroplasmavirus SVTS2, into chromosomes or extrachromosomal DNA of Spiroplasma (S.) citri can produce resistance to superinfection by SVTS2 in the offsprings. DNA fingerprinting and electrophoretic protein profiles of two second-generation SVTS2 resistant S. citri lines indicate that three proteins are missing in the resistant lines. Reaction with antiserum against S. citri membrane proteins suggests that the resistance is not because of virus receptors located on the surface.

Research, Analysis, Virus diseases, DNA testing, DNA identification, Mycoplasma, Superinfection

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Quality sample collection, handling, and preservation for an effective microbial forensics program

Article Abstract:

A series of meetings were held at the Banbury Center, Cold Spring harbor, NY to address specific issues for the enhancement of microbial forensic capability. The microbial forensic investigation and its success is dependent upon proper collection, handling and preservation of sample or physical evidence, therefore, efforts should be intensified in these areas to ensure capability for robust development, validation and reliability of microbial forensics to support the attribution of crimes.

Conferences, meetings and seminars, Microbiology, Forensic sciences

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Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods

Article Abstract:

Sequences within the cholera toxin operon from Vibrio cholerae 01 were selectively amplified by a rapid polymerase chain reaction method. Bacterial strains of V. cholerae were used toseed oysters, crabmeat, shrimp and lettuce and were washed with alkaline peptone water. DNA isolation was found to be unnecessary for the effective application of the method on a variety of food complexes.

Usage, Food, Vibrio cholerae, Food microbiology, Identification and classification, Polymerase chain reaction

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