Respiration of Pseudomonas fluorescens as a function of intracellular substrate concentration

Article Abstract:

The effects of intracellular substrate concentration on the rate of microbial growth and respiration were analyzed in Pseudomonas fluorescence cell cultures during short-term starvation-enrichment experiments. Analysis of Pseudomonas fluorescence respiration and substrate consumption in starvation-enrichment experiments indicated the dependence of respiration rate on cell density. Furthermore, the relationship between respiration and pool size was based on simple Michael-Menten kinetics.

Physiological aspects, Microbial growth, Microbial respiration

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Thiocarbamate herbicide-inducible nonheme haloperoxidase of Rhodococcus erythropolis NI86/21

Article Abstract:

The gene encoding the ThcF protein was cloned and sequenced from Rhodococcus erythropolis NI86/21 to determine the mechanisms that mediate the biodegradation of thiocarbamates. The thcF gene encoding the thiocarbamate-inducible protein F exhibited high GC content with a molecular mass of 29,664 kDa. The ThcF protein of the noncardioform Rhodococcus erythropolis also exhibited a protein sequence that was homologous to nonheme haloperoxidase.

Biodegradation, Microbial enzymes

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The non-haem chloroperoxidase from Pseudomonas fluorescens and its relationship to pyrrolnitrin biosynthesis

Article Abstract:

A chloroperoxidase (CPO) gene from the pyrrolnitrin producer Pseudomonas fluorescens BL914 was cloned. Its overexpression in Escherichia coli and the stability of CPO-F against higher temperatures and proteases showed that it was purified to homogeneity. Its partial characterization revealed that it is a member of the class of bacterial non-haem CPOs. Inactivation of the gene also showed that it has no role in pyrrolnitrin biosynthesis.

Analysis, Cloning

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