Reverse transcription-PCR differential display analysis of Escherichia coli global gene regulation in response to heat shock

Article Abstract:

Reverse transcription(RT)-PCR technique has been developed to analyze global gene regulation in Escherichia coli and it has been used for heat shock analysis to demonstrate validity. A novel combination of primers specifically designed for the start-and-stop regions of E. coli genes was used as an alternative to the poly(T) primers often used in eukaryotic RT-PCR. The method can easily be extended to study E. coli global gene regulation in response to more environmental stimuli.

United States, Statistical Data Included, Usage, Polymerase chain reaction, Microbiology, Heat shock proteins, Cytochemistry

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Characterization of an extremely thermostable restriction enzyme, PspGI, from a Pyrococcus strain and cloning of the PspGI restriction-modification system in Escherichia coli

Article Abstract:

The thermostable restriction enzyme PspGI and the cloning and expression of the pspGIR and psrGIM genes in Escherichia coli were described. The modification genes of PspGI contained the conserved sequence motifs of alpha-aminomethyltransferases which suggested that it must be an N4-cytosine methylase. PspGI was expressed in E. coli via a T7 expression system. Recombinant PspGI had a half-life of two hours at 95 degrees C. PspGI may be used in DNA-based diagnostic applications.

Gene expression, Restriction enzymes, DNA, DNA restriction enzymes

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Molecular analysis of the O-antigen gene cluster of Escherichia coli O86:B7 and characterization of the chain length determination gene (wzz)

Article Abstract:

The complete sequence of Escherichia coli O86:B7 O-antigen gene cluster between galF and hisI is reported to determine the genetic basis for the O-antigen differences between two O86 strains. The results have proved that the chain length determinant genes (wzz) are responsible for the different lipopolysaccharide (LPS) patterns found in the two O86 subtypes and also that the very short type of LPS is responsible for the serum sensitivity of the O86:B7 strain.

Petroleum Refineries, Petroleum refining, Polymerization, Analysis, Structure, Chemical properties, Bacterial antigens, O antigens

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