Ribosomal DNA internal transcribed spacers to estimate the proportion of Pisolithus tinctorius and Eucalyptus globulus RNAs in ectomycorrhiza

Article Abstract:

The steady-state levels of symbiosis-regulated messenger ribonucleic acid (mRNA) during the development of the ectomycorrhiza was analyzed by utilizing the ribosomal deoxyribonucleic acid (rDNA) as a molecular probe. Analysis of the cloned internal transcribed spacer (ITS)-5.85 rDNAs of Pisolithus tinctorius and Eucalyptus globulus indicated the presence of 5.8S rDNAs that did not cross-hybridize. Furthermore, the ITS-5.85 rDNAs were successfully used to the normalize transcript accumulation of fungal hydrophobins and plant alpha-tubulins during ectomycorrhiza formation.

Analysis, Physiological aspects, RNA, Mycorrhizas, Mycorrhizae, Eucalyptus

---

Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order Glomales

Article Abstract:

Analysis of a range of isolates from Glomus (G.) mosseae and G. fasciculatum indicated the presence of high sequence divergence in the internal transcribed spacers (ITS) of microbes belonging to the genus Glomus. Restriction fragment analysis of the ITS of three strains of Glomus indicated the presence of highly variable ITS sequences and compared to several species of Gigaspora which exhibited similar restriction patterns. Furthermore, the restriction patterns of G. manihotis and G. clarum were indistinguishable from each other.

Identification and classification, Microbiology, Taxonomy (Biology), Phylogeny

---

Genotypic characterization of Bradyrhizobium strains nodulating endemic woody legumes of the Canary Islands by PCR-restriction fragment length polymorphism analysis of genes encoding 16S rRNA (16S rDNA) and 16S-23S rDNA intergenic spacers, repetitive extragenic palindromic PCR genomic fingerprinting, and partial 16S rDNA sequencing

Article Abstract:

A study was conducted to examine the phylogenetic positions of the Canarian isolates and reference strains from the nodules of plants of the Chamaecytisus proliferus taxonomic complex and from other endemic woody Fabaceae using amplified rDNA restriction analysis. Genomic DNA was determined from liquid cultures while partial 16S rDNA sequences were amplified with primers. Results suggested that the taxonomy of the genus Bradyrhizobium be further developed in relation to isolates from tree or shrub legumes.

Plant genetics, Polymerase chain reaction, Gene expression, Ribosomal RNA, Legumes

Similar abstracts:

• Abstracts: Fungal degradation of lipophilic extractives in Eucalyptus globulus wood. Lignin-derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes
• Abstracts: Comparison of commercially available kits with standard methods for the detection of coliforms and Escherichia coli in foods
• Abstracts: Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water
• Abstracts: A Bacillus cereus member of the SNF2 family. Genome organization is not conserved between Bacillus cereus and Bacillus subtilis
• Abstracts: A 10.3 kbp segment from nprB to argJ at the 102-degree region of the Bacillus subtilis chromosome. Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.