Root nodule Bradyrhizobium spp. Harbor tfdA(alpha) and cadA, homologous with genes encoding 2,4-dichlorophenoxyacetic acid-degrading proteins

Article Abstract:

PCR and Southern hybridization methods were used for studying the distribution of tfdA(alpha) and cadA genes that encoded 2,4,dichlorohenoxyacetic acid-degrading proteins, which are the characteristic of the 2,4,-D-degrading Bradyrhizobium sp. isolated from pristine environments. The information that cadA genes of 2,4,-D-degrading and nondegrading Bradyhyrhizobium spp. may have different origins and could be obtained through the horizontal gene transfer was suggested by the Codon usage pattern and the GC content.

Codon, Codons, Genes

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Sensitive and specific molecular detection of Burkholderia pseudomallei, the causative agent of melioidosis, in the soil of tropical northern Australia

Article Abstract:

A direct soil Burkholderia pseudomallei DNA detection method is developed and evaluated based on the real-time PCR targeting the Burkholderia pseudomallei type III secretion system. The analysis of soil samples from tropical northern Australia has revealed significant association between Burkholderia pseudomallei-positive sites and the presence of animals at these locations and also with moist, reddish brown-to-reddish gray soils.

Australia, Physiological aspects, DNA testing, DNA identification, Saprophytes

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Identification and expression analyses of putative sesquiterpene synthase genes in Phormidium sp. and prevalence of geoA-like genes in a drinking water reservoir

Article Abstract:

Primers are designed for the identification of two similar genes in cyanobacterial species, classified as Phormidium sp., and their expression is studied by using reverse transcription-PCR (RT-PCR). The PCR fragments with the same degenerate primer pair are generated in order to analyze the prevalence of geoA1 and geoA2 in samples from the phylobenthos.

Genetic aspects, Cyanobacteria, Drinking water

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