Sampling natural viral communities from soil for culture-independent analyses

Article Abstract:

Four elution buffers and three enumeration techniques were compared to determine the best method of extracting autochthonous bacteriophages from two Delaware agricultural soils. Results suggest that measurement of phage abundance in soils varies with both the extraction and enumeration methodology, and hence it is important to assess multiple extraction and enumeration approaches prior to undertaking ecological studies of phages in a particular soil.

Analysis, Soil research, Combinatorial enumeration problems

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Assessment of factors influencing direct enumeration of viruses within estuarine sediments

Article Abstract:

Differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately processed onboard ship or frozen at -20 degrees Celsius and later processed are evaluated. No significant difference in viral abundance was apparent between extracts from fresh or frozen sediments, however, in contrast, bacterial abundance was significantly lower in the samples subjected to freezing.

Atlantic Ocean, Risk factors, Environmental aspects, Chesapeake Bay, Estuarine sediments, North Atlantic Ocean, Anaerobic infections

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Abundance and diversity of viruses in six Delaware soils

Article Abstract:

A study was conducted to describe the abundance and diversity of autochthonous viruses in six Delaware soils. Extracted soil virus communities were dominated by bacteriophages that demonstrated a wide range of capsid diameters and morphologies.

Biological diversity, Biodiversity, Morphological variation

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