Sequence and functional analysis of the Streptomyces phaeochromogenes plasmid pJV1 reveals a modular organization of Streptomyces plasmids that replicate by rolling circle

Article Abstract:

The pJV1 circular plasmid of the bacteria Streptomyces phaeochromogenes contains transfer genes needed for chromosome mobilization (Cma) during replication of the plasmid by rolling circle mechanism. These transfer genes are controlled by a repressor gene which is similar to genes of the GntR family of regulators. Uncontrolled expression of the transfer gene causes lethality. The plasmid also contains three genes which are not needed for Cma but increase spreading of the plasmid. The amino acid sequence for the plasmid is given.

Observations, Amino acid sequence, Amino acid sequencing

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Functional insights into pG12, a cryptic rolling-circle replicating plasmid from Bacillus thuringiensis

Article Abstract:

A detailed functional analysis of pG12, a 9672 bp plasmid from Bacillus thuringiensis H1.1 that harbors the 4149 bp transposon Tn4430, was conducted. The experiment included its double-strand and single-strand replication module, the activity of its Mob proteins and the influence of various modules on its segregational stability. The distribution of the pG12 replicon among members of the Bacillus cereus group was also investigated.

Bacillus thuringiensis, Viruses

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The conjugative plasmid pSG5 from Streptomyces ghanaensis DSM 2932 differs in its transfer functions from other Streptomyces rolling-circle-type plasmids

Article Abstract:

PSG5 is a Streptomyces ghanaensis plasmid which is self transmissible. Its complete nucleotide sequence has been determined and it was found that just one gene, traB is involved in pSG5 transfer. PSG5 transfer is not linked to pock formation, but it does have putative spd genes that result in pock phenotypes of other Streptomyces plasmids. Spd genes of pSG5 are not transcribed.

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