Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene

Article Abstract:

Species-specific primers and a multiplex PCR assay are developed for the simultaneous identification and differentiation of Pseudomonas fragi, Pseudomonas lundensis, and Pseudomonas putida, which is based on the coamplification of different portions of the small subunit of the carbomoyl phosphate synthase gene (carA). The carA multiplex PCR is used for detecting the presence of the three Pseudomonas species in meat and it is proved to be effective in showing their evolution during the storage of meat.

Analysis, Physiological aspects, Pseudomonas, Polymerase chain reaction, Nucleotide sequencing

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Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses

Article Abstract:

Thirty-five Staphylococcus aureus strains, including 10 reference strains and 25 strains recovered from clinical specimens and food samples, were analyzed by PCR restriction endonucleases analysis (REA) of the egc operum and spa typing. By an analysis of their nucleotide sequence identities, a new homogeneous cluster of a sei variant, called the sei variant, was detected in six strains.

Science & research, Staphylococcus aureus, Genetic polymorphisms

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Enterotoxigenic potential of Staphylococcus intermedius

Article Abstract:

Some strains of Staphylococcus intermedius contain the staphylococcal enterotoxin sec gene and could therefore produce enterotoxin. These strains were found in dogs, horses, and humans.

Staphylococcus

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