Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis

Article Abstract:

A single primer set and DNA intercalating dye SYTO9 were used for real-time PCR and melting-curve analysis of the 5.8 ribosomal DNA gene and flanking noncoding spacers (ITS1, ITS2) in a unique closed-tube technique to identify thermophilic Naegleria species like Naegleria fowleri, Naegleria italica and Naegleria australiensis. It was observed that at closed temperature intervals, the collection of DNA melting data demonstrated highly informative melting curves with one or more identifiable melting peaks, recognized seven Naegleria types and the related Willaertia magna.

Australia, Genetic aspects, Bacteria, Thermophilic, Thermophiles, Protista, Protists

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Culture-independent techniques for rapid detection of bacteria associated with loss of chloramine residual in a drinking water system

Article Abstract:

A study of water treatment plant (WTP) and distribution system employing chloramination during an instability event is conducted using flow cytometric cell sorting coupled with PCR-denaturing gradient gel electrophoresis (PCR-DGGE). No active bacteria was detected when water left the WTP, but 12 km downstream the chloramine residual had diminished and the level of active bacteria in the bulk water had increased to more than 1x10(super 5) bacteria ml(super -1).

Flow cytometry, Genetic research, Aliphatic acids

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Biodiversity of bacterial ecosystems in traditional Egyptian Domiati cheese

Article Abstract:

The PCR-temporal temperature gel electrophoresis (TTGE) and PCR-denaturing gradient gel electrophoresis (DGGE) was used to study the bacterial biodiversity occurring in traditional Egyptian soft Domiati cheese. The microbial composition of Domiati cheese reveal great biodiversity with 5.4 to 9.5% NaCl content and could play an important role in the ripening process and determining the role of the marine bacteria the cheese type.

Cheese, natural and processed, Cheese, Cheese Manufacturing, Physiological aspects, Gel electrophoresis, Marine bacteria, Properties

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