Single-site oxidation, cysteine 108 to cysteine sulfinic acid, in D-amino acid oxidase from Trigonopsis variabilis and its structural and functional consequences

Article Abstract:

A study shows that side chain of Cys108 is the main site undergoing stress-induced oxidation in Trigonopsis variabilis (TvDAO) D-amino acid oxidase and high-resolution anion-exchange chromatography is use to separate the reduced and oxidized protein forms. The results reveal unprecedented routes for the stabilization of TvDAO and provide insights into the structure-functional relationships as well as strongly emphasize the potential value of identifying the site of chemical modification in biocatalysts that are sensitive to oxidation.

Analysis, Yeast fungi, Yeasts (Fungi), Oxidation-reduction reaction, Oxidation-reduction reactions, Chemical properties, Adenylic acid, Adenosine monophosphate, Cysteine

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Engineering Candida tenuis xylose reductase for improved utilization of NADH: Antagonistic effects of multiple side chain replacements and performance of site-directed mutants under simulated in vivo conditions

Article Abstract:

Six single- and multiple-site variants of Candida tenuis xylose reductase that were engineered to have side chain replacements in the coenzyme 2'-phosphate binding pockets were tested for NADPH versus NADH selectivity (R(sub sel)) in the presence of physiological reactant concentrations. The experimental R(sub sel) values matched with the predictions from a kinetic mechanism describing mixed alternative coenzyme utilization.

NAD (Coenzyme), Nicotinamide adenine dinucleotide

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Mutatution of Candida tropicalis by irradiation with a He-Ne laser to increase its ability to degrade phenol

Article Abstract:

The biodegradation of phenol by Candida tropicalis is enhanced by exposing the organism to radiations from a He-Ne laser. The irradiations caused mutation of four amino acids in the phenol hydroxylase gene.

Environmental aspects, Irradiation, Microbial mutation, Irradiation (Radiation exposure)

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