Slicer function of Drosophila argonautes and its involvement in RISC formation

Article Abstract:

Argonaute proteins play important and distinct roles in RNA silencing. Studies show that upon addition of an siRNA duplex in S2 Iysate, the passenger strand is cleaved in an AGO2-dependent manner, and nuclease-resistant modification of the passenger strand impairs RISC formation and these findings give rise to a new model in which AGO2 is directly involved in RISC formation as Slicer of the passenger strand of the siRNA duplex.

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Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drasophila genome

Article Abstract:

A demonstration that endogenous Piwi immunopurified from ovary specifically associates with small RNAs of 25-29 nucleotides in length is presented. Piwi-associated small RNAs are identified by cloning and sequencing as repeat-associated small interfering RNAs (rasiRNAs) derived from repetitive regions, such as retrotransposon and heterochromatic regions in the Drasophila genome.

Iran, Retrotransposons

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Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi-interacting RNAs at their 3' ends

Article Abstract:

Piwi-interacting RNAs (piRNAs) which function in silencing selfish transposable elements through binding with PIWI proteins in Drosophila were studied. It was shown that piRNAs in Drosophila are 2'-O-methylated at their 3' ends and Pimet (piRNA methyltransferase) mediates piRNA 2'-O methylation in Drosophila.

Physiological aspects, DNA binding proteins, DNA-ligand interactions, DNA binding

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