Spatial distribution and stability of the eight microbial species of the altered schaedler flora in the mouse gastrointestinal tract

Article Abstract:

The spatial distribution, interanimal variation, and persistence of bacteria in the most complex defined-flora model system to date, that is- mice colonized with the eight strains of the altered schaedler flora (ASF) is determined. A comparison of the relative distribution of the ASF strains in feces and the colon indicated large differences, suggesting that fecal bacterial levels may provide a poor approximation of colonial bacterial levels.

Product information, Testing, Plants, Plants (Organisms), Microbiology, Gastrointestinal diseases, Mice as laboratory animals, House mouse

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Evaluation of 23S rRNA PCR primers for use in Phylogenetic studies of bacterial diversity

Article Abstract:

A study was conducted to incorporates data from large-scale sequencing efforts to develop new and evaluate existing bacterium-specific 23S rRNA PCR amplification primers. A comparison of 23S and 16S rRNA gene clone libraries constructed from replicate water samples yielded gross similarities but also some important differences.

Microbial populations

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PCR-induced sequence artifacts and bias: Insights from comparison of two 16S rRNA clone libraries constructed from the same sample

Article Abstract:

The contribution of PCR artifacts to 16S rRNA gene sequence diversity from a complex bacterioplankton sample is estimated. The recommendations are provided for modification of amplification protocols and for reporting diversity estimates at 99% sequence similarity as a standard.

Genetic aspects, DNA polymerases, Planktonic bacteria

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