Antisense PNA effects in Escherichia coli are limited by the outer-membrane LPS layer

Article Abstract:

Antisense peptide nucleic acid (PNA) effects in Escherichia coli have been found to be limited by the outer-membrane LPS layer. PNA potency was better in wild-type cells grown in the presence of certain cell-wall -permeabilizing agents. PNAs that can enter E. coli likely remain active inside cells. To study factors that limit PNA antisense effects in isolation from nonspecific inhibition, a PNA that would target the lac repressor mRNA was designed.

Denmark, Sweden, Cells, Bacterial cell walls, Cell permeability, Messenger RNA, Antisense drugs

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Streptococcus pyogenes scIB encodes a putative hypervariable surface protein with a collagen-like repetitive structure

Article Abstract:

Streptococcus pyogenes scIB has been found to encode a putative hypervariable surface protein with a repetitive structure that is collagen-like. An ORF has been identified during a screen of the genomic sequence potentially encoding the hypervariable surface protein. The ORF has been shown to be highly variable and to have short sequence repeats (SSRs) just downstream of the putative start codon that perhaps regulates expression of the corresponding SclB protein product.

Membrane proteins, Streptococcus pyogenes, Variation (Biology)

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Differential expression of mycobacterial proteins following phagocytosis by macrophages

Article Abstract:

Mycobacterial proteins differentially expressed after phagocytosis by macrophages have been studied. Mycobacterium tuverculosis stays inside the macrophages of the host, but molecular and cellular mechanisms of survival have not been adequately studied. Identifying proteins by a method discussed may help to find the molecular basis of attenuation and vaccine potential of BCG and may find antigens that distinguish infection with M. tuberculosis from vaccination with BCG. The patterns of gene expression of Mycobacterium bovis BCG inside the human leukemic macrophage cell line THP-1 have been defined. An in vitro macrophage infection model that permits the recovery of intracellular bacilli free from contaminating host cell proteins has been developed.

Tuberculosis, Macrophages, Host-bacteria relationships, Gene expression, Mycobacteria, Mycobacterium, BCG vaccination

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