Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway

Article Abstract:

Wnt signals have been shown to be required and sufficient for neural crest cells to adopt pigment cell fates. A zebrafish homolog of microphthalmia-associated transcription factor (MITF) called nacre is needed for differentiation of pigment cells. A promoter region of nacre that has in it Tcf/Lef binding sites, which can mediate Wnt responsiveness were, isolated. The promoter binds to Lef1 protein of zebrafish in vitro. In melanoma cells, a nacre reporter construct is repressed strongly by dominant-negative Tcf. Mutation of Tcf/Lef sites gets rid of Lef1 binding and reporter function in vivo.

Physiological aspects, Organic pigments, Homology (Biology), Cell differentiation, Neural crest, Binding sites (Biochemistry), Active sites (Biochemistry), Melanoma, Melanocytes

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The zebrafish bonnie and clyde gene encodes a Mix family homeodomain protein that regulates the generation of endodermal precursors

Article Abstract:

A Mix family homeodomain protein that regulates generation of endodermal precursors is encoded by the zebrafish bonnie and clyde (bon) gene. That bon encodes a paired-class homeodomain protein of the Mix family expressed transiently before and during early gastrulation in mesodermal and endodermal progenitors has been shown. Endodermal gene expression and the formation of a gut tube in bon mutants can be rescued by overexpression of bon. It has been shown as well by loss- and gain-of function analyses that Bon functions only downstream of squint and cyclops signaling.

Homeobox genes, Gastrointestinal system

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A large-scale insertional mutagenesis screen in zebrafish

Article Abstract:

A strategy for the most efficient breeding and screening of proviral insertions has been developed to make a large-scale insertional mutagenesis screening possible. Such a screen is being carried out in zebrafish, in the embryo of which about 2500 genes are essential for normal development. The screen is to isolate about 1000 embryonic mutations. A significant proportion of the mutated genes, those important for normal embryogenesis of a vertebrate, are to be cloned. The first mutants obtained and the rapid cloning of the mutated genes are discussed.

Statistical Data Included, Methods, Cloning, Embryology, Experimental, Embryological research, Genetic screening, Genetic testing, Genetic vectors, Animal mutation

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