Structural and functional organization of the Yersinia pestis bacteriocin pesticin gene cluster

Article Abstract:

The primary structure and organization of the Yersinia pestis bacteriocin pesticin gene cluster, which includes genes encoding pesticin activity and immunity proteins and potentially a lysis peptide, are described. Specifically, the primary structure of a 2671 bp DNA fragment between the pla gene and the origin of replication of the wild-type Y. pestis plasmid pYP358 were determined. The arrangement of the pesticin gene cluster of Y. pestis plasmid pYP358 resembles that of the genes of the pore-forming colicins A, B, E1, Ib, Ia, K and N and of colicin M.

Plasmids, Yersinia pestis

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The gene cluster directing O-antigen biosynthesis in Yersinia enterocolitica serotype O:8:identification of the genes for mannose and galactose biosynthesis and the gene for the O-antigen polymerase

Article Abstract:

The molecular genetic analysis of the O-antigen of Yersinia enterocolitica serotype O:8 (YeO8) is examined. Cosmid cloning was used to clone the rfb gene cluster of Y. enterocolitica serotype O:8 (YeO8) strain 8081-c. The analysis showed that the rfb gene cluster of YeO8 is found between the adk-hemH and gsk loci. The order is adk-hemH-rfb-rfc-gsk in the chromosome. The locus downstream of the hemH gene is occupied by gene clusters involved in LPS biosynthesis, as in other Yersinia spp.

Research, Biosynthesis, Transposons, Mutagenesis, Yersinia enterocolitica

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