Structural and kinetic properties of nonglycosylated recombinant Penicillium amagasakiense glucose oxidase expressed in Escherichia coli

Article Abstract:

Research was conducted to examine the structural and kinetic properties of nonglycosylated recombinant Penicillium amagasakiense glucose oxidase (GOX) expressed in Escherichia coli. Recombinant GOX activity, expressed in the form of insoluble inclusion bodies, was reconstituted and the active enzyme was proven to possess properties and secondary structure composition similar to P amagasakiense GOX. Results demonstrate that the protein-bound carbohydrate moiety is not important for the correct folding of GOX.

Genetic aspects, Escherichia coli, Enzyme activation

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Degradation of perennial ryegrass leaf and stem cell walls by the anaerobic fungus Neocallimastix sp. strain CS3b

Article Abstract:

The anaerobic fungus Neocallimastix sp. strain CS3b degrades almost all the glucose, xylose, and arabinose, and some galactose from the leaf and stem cell walls of perennial ryegrass. The degradation of sugars from the leaf cell walls is faster than that from the stem cell walls. The complete growth of the fungus strain occurs in 4 days in culture and 95% of the fermentable sugars are released. Neocallimastix sp. strain CS3b is present in the rumen of ruminants such as sheep.

Physiological aspects, Fungi, Sugars, Plant cell walls, Cell walls

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Trehalose synthesis by sequential reactions of recombinant maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase from Brevibacteruim helvolum

Article Abstract:

Researchers describe the production of trehalose using two fused enzymes, maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase. The resulting fused enzyme could product trehalose from starch, which could be an abundant source of trehalose in the food, cosmetic, and pharmaceutical industries.

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