Structural stability of Bacillus thuringiensis delta-endotoxin homolog-scanning mutants determined by susceptibility to proteases

Article Abstract:

A study of 40 homolog-scanning mutant proteins of two Bacillus thuringiensis delta-endotoxin genes revealed three mutant groups. Group1 with 13 mutants did not contain delta-endotoxin under overexpressed conditions in Escherichia coli. Group 2 comprised 13 mutants that produced delta-endotoxins that were fully digested by 2% bovine trypsin. Group 3 mutants revealed the presence of delta-endotoxin and trypsin-stable toxins similar to wild-type toxins.

---

Role of tryptophan residues in toxicity of Cry1Ab toxin from Bacillus thuringiensis

Article Abstract:

A study was conducted to examine the role of Trp residues in the structure and function of Cry1Ab toxin, produced by Bacillus thuringiensis. The results show that W65 and W316 are important for insecticidal activity of the toxin since their replacement by Phe reduced toxicity against Manduca sexta.

Physiological aspects, Tryptophan

---

Enhancement of cry19Aa mosquitocidal activity against aedes aegypti by mutations in the putative loop regions of domain II

Article Abstract:

It is shown in the study that cry19Aa in bacillus thuringiensis is enhanced in toxicity for aedes aegypti. This is the first time toxicity in a cry toxin is increased by protein engineering.

Similar abstracts:

• Abstracts: Regulation of the packaging of Bacillus thuringiensis delta-endotoxins into inclusions. Bacillus thuringiensis delta-endotoxin Cry1 hybrid proteins with increased activity against the Colorado potato beetle
• Abstracts: Binding of the CryIVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins. High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20 kilodalton protein, and the coexprssed gene products are synergistic in their toxicity to mosquitoes
• Abstracts: Integrative model for binding of Bacillus thuringiensis toxins in susceptible and resistant larvae of the diamondback moth (Plutella xylostella)
• Abstracts: Two different Bacillus thuringiensis delta-endotoxin receptors in the midgut brush border membrane of the European corn borer, Ostrinia nubilalis (Hubner)(Lepidoptera: Pyralidae)
• Abstracts: Genetic instability and strain degeneration in Streptomyces rimosus. Genetics of Streptomyces rimosus, the oxytetracycline producer

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.