Structure and function of fas-1A, a gene encoding a putative fatty acid synthetase directly involved in aflatoxin biosynthesis in Aspergillus parasiticus

Article Abstract:

The A. parasiticus fas-1A gene encodes a fatty acid synthetase that produces a part of the polyketide backbone of aflatoxin B1 (AFB1). The gene is cloned by genetic complementation of an A. parasiticus mutant strain with blockages in the AFB1 biosynthetic pathway. The disruption of the fas-1A gene sequence reduces norsolorinic acid formation and increases sclerotium development. The amino acid sequence of the fas-1A gene is similar to the enoyl reductase, and malonyl/palmityl transferase functional regions in the beta-subunit of yeast FAS.

Observations, Ligases, Amino acid sequence, Amino acid sequencing

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Physical and transcriptional map of an aflatoxin gene cluster in 'Aspergillus parasiticus' and functional disruption of a gene involved early in the aflatoxin pathway

Article Abstract:

The genes residing in the nor-1 and ver-1 region are used to synthesize aflatoxin B1 (AFB1) in Aspergillus parasiticus. In A. parasiticus CS10, which contains the aflatoxin precursor versicolorin A, the function of gene-1 encoding one of the eight transcripts is inactivated. Analysis indicate that gene-1 disruptant clones are not able to accumulate versicolorin, which confirms the role of gene-1 in aflatoxin synthesis.

Biosynthesis

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New additive for culture media for rapid identification of aflatoxin-producing Aspergillus strains

Article Abstract:

Adding a cyclodextrin to culture media can be used to detect the presence of Aspergillus. After a few days' incubation, the production of aflatoxin leads to a bight blue or blue-green fluorescence around fungal colonies that can be seen under UV light.

Culture media (Biology), Composition, Culture media

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