Subcellular location of XpsD, a protein required for extracellular protein secretion by Xanthomonas campestris pv. campestris

Article Abstract:

The last ORF of the xps gene cluster, xpsD, encodes the XpsD protein which is an outer membrane protein and a part of the XpsD sequence is exposed to the cell surface. XpsD is fatty acylated but its position on the outer membrane and secretory function does not depend on this fatty acylation. A mutation in the N-terminal lipoprotein signal peptide of the amino acid sequence produces a non-fatty acylated protein whose secretory activity is unaffected. However a C-terminal truncated protein formed by a mutated xpsD gene is incapable of encoding the extracellular proteins.

Gram-negative bacteria

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Use of green fluorescent protein for detection of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis

Article Abstract:

Wild-type and mutant forms of the gene encoding green fluorescent protein (GFP) from Aequorea victoria were inserted into Bacillus subtilis as translational fusions to the prespore-specific and mother-cell-specific genes dacF and spoIVA. The protein was easily detected by fluorescence microscopy and its synthesis was correctly localized. A mutation of the gfp gene that alters the light emitted by the protein from green to blue cannot be utilized because of the intrinsic blue-colored autofluorescence of B. subtilis.

Usage, Bacillus subtilis, Fluorescence microscopy

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Insertion mutagenesis of XpsD, an outer membrane protein involved in extracellular protein secretion in Xanthomonas campestris pv. campestris

Article Abstract:

A study was conducted to characterize the structure of XpsD proteins by examining semi-randomly inserted linker-insertion mutant XpsD proteins. Insertion sites in the mutant proteins were randomly distributed throughout the XpsD protein's sequence. Target DNA was carried out from the mutant xpsD gene by polymerase chain reaction. Results indicated conformational alterations in XpsD that were too subtle to be identified by other assays.

Genetic aspects, Membrane proteins, Mutagenesis, Insertion elements, DNA, DNA insertion elements

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