Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement

Article Abstract:

The 70S ribosome changes from a compact structure to a non-compact structure affecting the arrangement of proteins as revealed by cryo-electron microscopy density map real-space refinement analysis. The briges formed by proteins exhibit conformational changes upon a ratchet-like motion. Data indicate that the 70S ribosome of Escherichia coli presents two different functional states.

Science & research, Research, Escherichia coli, Conformational analysis

---

Structure of the mammalian mitochondrial ribosome reveals an expanded functional role for its component proteins

Article Abstract:

A three-dimensional cryo-electron microscopic analysis of the mammalian mitochondrial 55S ribosome carrying a tRNA at its P site show many proteins localized to new positions and the ribosome exhibits inter-subunit bridges consisting mainly proteins. Data show a gatelike structure at the mRNA entrance and a polypeptide exit tunnel.

Mitochondria, Ribosomal proteins

---

Locking and unlocking of ribosomal motions

Article Abstract:

The rachet-like rotation of the 30S subunit with respect to the 50S ribosome subunit, during the ribosomal translocation, involves a 20 angstrom movement of the L1 stalk of the 50S subunit. Data indicate that the ribosomal motions requires deacylation of the P-site tRNA, which upon charging locks the ribosome.

Sweden, Influence, Protein synthesis, Protein biosynthesis, Transfer RNA, Protein conformation

Similar abstracts:

• Abstracts: The structure and evolution of subtelomeric Y' repeats in Saccharomyces cerevisiae. Site-specific recombination determined by I-SceI, a mitochondrial group I inton-encoded endonuclease expressed in the yeast nucleus
• Abstracts: Structural changes in the antennapedia complex of Drosophila pseudoobscura. Ectopic expression of the Drosophila homeotic gene proboscipedia under Antennapedia P1 control causes dominant thoracic defects
• Abstracts: Construction of Escherichia coli strains for conversion of nitroacetophenones to ortho-aminophenols. Bacterial conversion of hydroxylamino aromatic compounds by both lyase and mutase enzymes involves intramolecular transfer of hydroxyl groups
• Abstracts: Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. BMP induction if Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
• Abstracts: Promoter cleavage: A Topollbeta and PARP-1 collaboration. NAD+- dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.